Culross ('holly point or promontory') is a village and former royal burgh, and parish, in Fife, Scotland.

According to the 2006 estimate, the village has a population of 395. Originally, Culross served as a port city on the Firth of Forth and is believed to have been founded by Saint Serf during the 6th century.

The civil parish had a population of 4,348 in 2011.

A legend states that when the Brittonic princess (and future saint) Teneu, daughter of the king of Lothian, became pregnant before marriage, her family threw her from a cliff. She survived the fall unharmed, and was soon met by an unmanned boat. She knew she had no home to go to, so she got into the boat; it sailed her across the Firth of Forth to land at Culross where she was cared for by Saint Serf; he became foster-father of her son, Saint Kentigern or Mungo.

The parish appears to have originally centred further west. The original church, later known as the "West Kirk" perhaps dates to the 11th century but was abandoned around 1500 and therefore did not come into play in 1560 at the time of the Reformation. However, it continued to be used for burials into the 20th century, being a long-established burial ground.

Meanwhile the Cistercian Abbey dedicated to the Virgin Mary and St. Serf was built around a mile to the east in 1217, being founded by Malcolm, Earl of Fife. Part of this became the parish church in 1560 and was restored in 1905. A Chapel of St. Mungo (now wholly lost) was erected in 1503 by Robert Blackadder, Archbishop of Glasgow.

The first recorded minister was John Dykes (1567), He was replaced by Robert Colville of Linlithgow in 1593 who ministered until 1629 when replaced by his assistant Robert Melville. John Duncan MA took over in 1632. Duncan was pensioned off in 1642 but is recorded as joining the camp of General Leslie at Newcastle 1646/7 during the English Civil War.

In the 17th century its most famous minister was the Covenanter James Fraser of Brea who took over in 1689.

Notable buildings in the burgh include Culross Town House, formerly used as a courthouse and prison, the 16th century Culross Palace, 17th century Study, and the remains of the Cistercian house of Culross Abbey, founded 1217. The tower, transepts and choir of the Abbey Church remain in use as the parish church, while the ruined claustral buildings are cared for by Historic Environment Scotland.

The West Kirk fell out of use before 1633 when it was noted as no longer serving as the parish church. The West Kirk was also the site where four women executed for witchcraft in 1675 were alleged to have congregated.

Just outside the town is the 18th-century Dunimarle Castle, built by the Erskine family to supersede a medieval castle.

Thomas Cochrane, 10th Earl of Dundonald spent much of his early life in Culross, where his family had an estate. A bust in his honour by Scott Sutherland can be seen outside the Culross Town House. He was the first Vice Admiral of Chile.

The war memorial was erected in 1921 to a design by Sir Robert Lorimer.

During the 20th century, it became recognised that Culross contained many unique historical buildings and the National Trust for Scotland has been working on their preservation and restoration since the 1930s.