Tuscany is a region in central Italy with an area of about 23,000 square kilometres (8,900 square miles) and a population of about 3.8 million inhabitants. The regional capital is Florence (Firenze). Tuscany is known for its landscapes, history, artistic legacy, and its influence on high culture. It is regarded as the birthplace of the Italian Renaissance and has been home to many figures influential in the history of art and science, and contains well-known museums such as the Uffizi and the Pitti Palace. Tuscany is also known for its wines, including Chianti, Vino Nobile di Montepulciano, Morellino di Scansano, Brunello di Montalcino and white Vernaccia di San Gimignano. Having a strong linguistic and cultural identity, it is sometimes considered "a nation within a nation". Tuscany is the second most popular Italian region for travellers in Italy. The main tourist spots are Florence, Pisa, Castiglione della Pescaia, Grosseto and Siena. The town of Castiglione della Pescaia is the most visited seaside destination in the region, with seaside tourism accounting for approximately 40% of tourist arrivals. The Maremma region, Siena, Lucca, the Chianti region, Versilia and Val d'Orcia are also internationally renowned and particularly popular spots among travellers. Seven Tuscan localities have been designated World Heritage Sites: the historic centre of Florence (1982); the Cathedral square of Pisa (1987); the historical centre of San Gimignano (1990); the historical centre of Siena (1995); the historical centre of Pienza (1996); the Val d'Orcia (2004), and the Medici Villas and Gardens (2013). Tuscany has over 120 protected nature reserves, making Tuscany and its capital Florence popular tourist destinations that attract millions of tourists every year. In 2018, the city of Florence was the world's 51st most visited city, with over 5 million arrivals.

Umbria is bordered by Tuscany to the west and the north, Marche to the east and Lazio to the south. Partly hilly and mountainous, and partly flat and fertile owing to the valley of the Tiber, its topography includes part of the central Apennines, with the highest point in the region at Monte Vettore on the border of the Marche, at 2,476 metres (8,123 feet); the lowest point is Attigliano, 96 metres (315 feet). It is the only Italian region having neither a coastline nor a common border with other countries. The comune of Città di Castello has an exclave named Monte Ruperto within Marche. Contained within Umbria is the hamlet of Cospaia, which was a tiny republic from 1440 to 1826, created by accident. Umbria is crossed by two valleys: the Umbrian valley ("Valle Umbra"), stretching from Perugia to Spoleto, and the Tiber Valley ("Val Tiberina"), north and west of the first one, from Città di Castello to the border with Lazio. The Tiber River forms the approximate border with Lazio, although its source is just over the Tuscan border. The Tiber's three principal tributaries flow southward through Umbria. The Chiascio basin is relatively uninhabited as far as Bastia Umbra. About 10 kilometres (6 miles) farther on, it joins the Tiber at Torgiano. The Topino, cleaving the Apennines with passes that the Via Flaminia and successor roads follow, makes a sharp turn at Foligno to flow NW for a few kilometres before joining the Chiascio below Bettona. The third river is the Nera, flowing into the Tiber further south, at Terni; its valley is called the Valnerina. The upper Nera cuts ravines in the mountains; the lower, in the Tiber basin, has created a wide floodplain. In antiquity, the plain was covered by a pair of shallow, interlocking lakes, the Lacus Clitorius and the Lacus Umber. They were drained by the Romans over several hundred years. An earthquake in the 4th century and the political collapse of the Roman Empire resulted in the refilling of the basin. It was drained a second time, almost a thousand years later, during a 500-year period: Benedictine monks started the process in the 13th century, and the draining was completed by an engineer from Foligno in the 18th century. The eastern part of the region, being crossed by many faults, has been often hit by earthquakes: the last ones have been that of 1997 (which hit Nocera Umbra, Gualdo Tadino, Assisi and Foligno) and those of 2016 (which struck Norcia and the Valnerina). In literature, Umbria is referred to as Il cuore verde d'Italia or The green heart of Italy. The phrase is taken from a poem by Giosuè Carducci, the subject of which is the source of the Clitunno River in Umbria.

Marche is one of the twenty regions of Italy. The name of the region derives from the plural name of marca, originally referring to the medieval March of Ancona and nearby marches of Camerino and Fermo. The region is located in the Central area of the country, bordered by Emilia-Romagna and the republic of San Marino to the north, Tuscany to the west, Umbria to the southwest, Abruzzo and Lazio to the south and the Adriatic Sea to the east. Except for river valleys and the often very narrow coastal strip, the land is hilly. A railway from Bologna to Brindisi, built in the 19th century, runs along the coast of the entire territory. Inland, the mountainous nature of the region, even today, allows relatively little travel north and south, except by twisting roads over the passes. Urbino, one of the major cities of the region, was the birthplace of Raphael, as well as a major center of Renaissance history.

Lazio is one of the 20 administrative regions of Italy. Situated in the central peninsular section of the country, it has 5,864,321 inhabitants - making it the second most populated region of Italy (after Lombardy and just ahead of Campania) - and its GDP of more than €197 billion per year means that it has the nation's second largest regional economy. The capital of Lazio is Rome, which is also the capital and largest city of Italy. Lazio has borders with Tuscany, Umbria, and Marche to the north, Abruzzo and Molise to the east, Campania to the south, and the Tyrrhenian Sea to the west. The region is mainly flat, with small mountainous areas in the most eastern and southern districts. The coast of Lazio is mainly composed of sandy beaches, punctuated by the headlands of Circeo (541 m) and Gaeta (171 m). The Pontine Islands, which are part of Lazio, are off Lazio's southern coast. Behind the coastal strip, to the north, lies the Maremma Laziale (the continuation of the Tuscan Maremma), a coastal plain interrupted at Civitavecchia by the Tolfa Mountains (616 m). The central section of the region is occupied by the Roman Campagna, a vast alluvial plain surrounding the city of Rome, with an area of approximately 2,100 km2 (811 sq mi). The southern districts are characterized by the flatlands of Agro Pontino, a once swampy and malarial area, that was reclaimed over the centuries. The Preapennines of Latium, marked by the Tiber valley and the Liri with the Sacco tributary, include on the right of the Tiber, three groups of mountains of volcanic origin: the Volsini, Cimini and Sabatini, whose largest former craters are occupied by the Bolsena, Vico and Bracciano lakes. To the south of the Tiber, other mountain groups form part of the Preapennines: the Alban Hills, also of volcanic origin, and the calcareous Lepini, Ausoni and Aurunci Mountains. The Apennines of Latium are a continuation of the Apennines of Abruzzo: the Reatini Mountains with Terminillo (2,213 m), Mounts Sabini, Prenestini, Simbruini and Ernici which continue east of the Liri into the Mainarde Mountains. The highest peak is Mount Gorzano (2,458 m) on the border with Abruzzo.

Abruzzo or Abruzzi is a region of Southern Italy with an area of 10,763 square km (4,156 sq mi) and a population of 1.3 million. It is divided into four provinces: L'Aquila, Teramo, Pescara, and Chieti. Its western border lies 80 km (50 mi) east of Rome. Abruzzo borders the region of Marche to the north, Lazio to the west and south-west, Molise to the south-east, and the Adriatic Sea to the east. Geographically, Abruzzo is divided into a mountainous area in the west, which includes the Gran Sasso d'Italia, and a coastal area in the east with beaches on the Adriatic Sea. Abruzzo is considered a region of Southern Italy in terms of its culture, language, history and economy, although geographically it may also be considered central. The Italian Statistical Authority (ISTAT) also deems it to be part of Southern Italy, partly because of Abruzzo's historic association with the Kingdom of the Two Sicilies. Abruzzo is known as "the greenest region in Europe" as almost half of its territory, the largest in Europe, is set aside as national parks and protected nature reserves. There are three national parks, one regional park, and 38 protected nature reserves. These ensure the survival of 75% of Europe's living species, including rare species such as the golden eagle, the Abruzzo (or Abruzzese) chamois, the Apennine wolf and the Marsican brown bear. Abruzzo is also home to Calderone, Europe's southernmost glacier. The visiting nineteenth-century Italian diplomat and journalist Primo Levi (1853-1917) said that the adjectives "forte e gentile" (strong and kind) best describe the beauty of the region and the character of its people. "Forte e gentile" has since become the motto of the region and its inhabitants.