Mikulov is a town in Breclav District in the South Moravian Region of the Czech Republic. It has about 7,500 inhabitants. The historic centre of Mikulov is well preserved and historically significant and is protected by law as urban monument reservation.

The town lies in the historic land of Moravia, located directly on the border with Lower Austria. In the south, a road border crossing leads to the neighbouring Austrian municipality of Drasenhofen. The highway is part of the European route E461 and is to be extended as the D52 motorway.

Mikulov is situated between the Pavlovské vrchy hilly area and the edge of the Mikulov Highlands, stretching up to the Thaya river and the three Nove Mlyny reservoirs. The Palava Protected Landscape Area begins in Mikulov, and so does the Moravian Karst.