Harlington is a village and civil parish located in Bedfordshire, England, near the M1 motorway. The nearest town is Flitwick about 3 miles (4.8 km) to the north.

English Heritage, in their listing notice, ascribe Harlington Manor to the 16th century, though recent architectural and documentary examination examination strongly suggests that the house dates, in fact, to the late 14th century and, possibly, to 1396. The Little Parlour contains obviously original, trestle sawn joists. Trestle sawing, as a technique, ceased to be used after the mid-1400's-putting the latest constructional date no later than that time. The house was owned by the Burwell Family of Virginia, from around 1500, but it passed, through intermarriage, to the Wingate family in the early 17th Century. It was, the listing notice claims, owned by Edmund Wingate, mathematician and tutor to Queen Henrietta Maria.

Famously, John Bunyan, the English divine, was interrogated by Sir Francis Wingate and briefly imprisoned in the house, in November 1660. Bunyan was sent to Bedford gaol where, over the next 12 years, he wrote The Pilgrim's Progress. It is thought that Harlington Manor is the only building still standing at which Bunyan is known to have stayed.

Charles II is said to have stayed briefly at the house in late 1660, apparently to thank Sir Francis Wingate for his help in dealing with the potential sedition of John Bunyan.

Harlington Manor was formerly known as Harlington House, its name being changed at some point towards the end of the 19th century. The house is private, but intermittently open to the public by private arrangement, or by "Invitation to View". Its exterior possesses a blue plaque, part of the Harlington Heritage Blue Plaque Scheme, commemorating the appearance of John Bunyan at the house.

Harlington Manor operates as an upmarket B and B, and was rated as one of the top three B and B's in the UK by the London Times.