Wyboston is a village in the English county of Bedfordshire. The eastern part of the village is dominated by the A1 Great North Road which at this point also carries the traffic of the A428 east–west road. The northern junction of these roads is grade-separated, while the southern junction is a roundabout. This causes frequent delays at the junction, and to users of both routes.

Wyboston is in the civil parish of Wyboston, Chawston and Colesden.

The remainder of Wyboston is horticultural and agricultural as a result of the proximity of the River Great Ouse.

Wyboston Lakes Resort is located on the edge of the village, and includes a golf course, hotel, spa, serviced offices and conference & training facilities. Wyboston is also the location of a conference centre owned and operated by Cambridge University's Robinson College. There is a public house 'Wait for the Waggon', and a service station which includes a BP garage with a Londis shop attached, Subway and a 24-hour McDonald's.